When CBT cells were treated with low temperature for 3days,flow cytometry revealed the hypodiploid apex and the apoptotic rate was shown to be 13%. 低温处理3 d的CBT细胞经流式细胞仪检测出现亚二倍体峰;凋亡率为13%25.
Methods Quantification of hypodiploid cell by flow cytometric analysis,analysis of DNA fragmentation were employd to detect cell apoptosis. 方法 DNA片段化分析、流式细胞仪分析测定亚二倍体细胞含量以检测淋巴细胞凋亡。