Hypoxanthine guanine Phosphoribosyl Transferase(HPRT) is a classical gene locus in the research of single gene mutation. 次黄嘌呤鸟嘌呤磷酸核糖转移酶(HPRT)基因在单基因突变的研究中是一个经典的基因位点。
The content of hypoxanthine in Hirudo was measured by high performance liquid chromatography(HPLC)and Hirudin was determined by thrombin. 以凝血酶法测定水蛭素的含量;以HPLC测定次黄嘌呤的含量。
METHODS: Cultured bovine RPE cells were erected, were divided into control group, hypoxanthine ( HX )/ xanthine oxidase ( XO ) Rg1 and Rg1 group ( HX/XO ), and Vitamin E group. 方法：建立牛RPE细胞氧化损伤的模型，并分为正常对照组（1mlDMEM培养液），次黄嘌呤（hypoxanthine，HX）/黄嘌呤氧化酶（xanthineoxidase，XO）损伤组（HX/XO组），Rb1组，Rg1组和维生素E组。
Microdialysis sampling coupled with high performance liquid chromatography and amperometric detector was developed and applied to determine Xanthine and Hypoxanthine in rat striatum. 采用微渗析活体取样技术和高效液相色谱安培检测法 ,测定了鼠脑纹状体中的黄嘌呤和次黄嘌呤。
An immobilization of xanthine oxidase on the surface of microelectrode has been accomplished, and thin film microsensor for the determination of hypoxanthine has been developed in this study. 本项研究实现在微电极表面进行黄嘌呤氧化酶的固定化,从而制备一体化薄膜次黄嘌呤微传感器。
Using hypoxanthine as starting material, a new bitterness blocker, adenosine 5'-monophosphate sodium salt, was prepared by chlorination, condensation, ammonolysis, phosphorylation and neutralization. 以次黄嘌呤为原料;经氯代、缩合、氨解、磷酸化、中和五步反应制备新型苦味屏蔽剂5’-腺苷单磷酸钠;总收率达37.;0%25。
