With immunohistology method, we detect the expression of Fas and FasL protein in SMMC-7721 cell. 免疫组织化学法检测SMMC-7721肝癌细胞胞膜及胞浆内Fas及FasL蛋白的表达变化。
PCR showed that recombinant adenoviruses contained EWS/FLI1 cDNA after the PBMC were transfected by Ad EWS/FLI1,the EWS/FLI1 mRNA and its expression were confirmed by RT-PCR and immunohistology. 同源重组后产生的pADEWS/FLI1经PCR鉴定构建成功 ,纯化后的滴度为 4× 1 0 10 /ml。 转染PBMC后 ,RT -PCR证实有EWS/FLI1mRNA的转录 ,免疫检测法检测在PBMC中有EWS/FLI1的表达。
Manual Of Diagnostic Antibodies For Immunohistology, 免疫学诊断抗体手册
Keywords Sclerosing Hemangioma of Lung;immunohistology;Oncopathology;imaging; 关键词硬化性血管瘤;肺;免疫组织化学;影像学;
