The 3H-thymidine target cells (YAC-1, P815, LAC-1, murine lymphoblast) were incubated with effector cells for 4 or 18 hr. The cultured cells were treated with trypsin and DNase. Then the cells were harvested by MASH and detected by liquid scintillator. 本文用 ~3H-胸腺嘧啶核苷(~3H-TdR)标记多种靶细胞(YAC-1、P815、LAC-1、小鼠淋巴母细胞)与不同效应细胞培养4或18小时,再用胰酶和DNA 酶处理靶细胞,收集细胞,用液体闪烁微杯法测定小鼠特异性和非特异性淋巴细胞介导的细胞毒试验,均获得良好结果。
The immunoconjugation of anti-T lymphoblast monoclonal antibody and daunomycin (DM) could selectively kill T lymphoblast CEM in vitro and did not show any effect on the non-T lymphocytes, such as K562, Raji and SP2/0, et al. 抗T淋巴母细胞单克隆抗体与柔红霉素的结合物,在体外对T淋巴母细胞CEM显示出选择性杀伤作用,而对K562、Raji、SP2/0等非T淋巴细胞则无杀伤效应。
Methods: To apply volatile oil of cortex mori and CY's reagents in mice after dividing them at random.According to the design, sample blood and dye;then observe lymphoblast and transitional cell. 方法:将小鼠随机分组后分别应用桑白皮挥发油等试剂,按设计取血染色,并观察淋巴母细胞的淋巴细胞和过渡型细胞。
Epstein-Barr virus-infected lymphoblast cell line 爱泼斯坦-巴尔病毒感染的淋巴母细胞系