Rhizogenesis--a morphogenetic event characterized by the appearance of root-bearing clumps of tissue--occurred. 根系发生--以出现带有成丛组织的根为特点的形态发生活动--产生了。
Proteins expressed during rhizogenesis of walnut cotyledon were profiled with the techniques of proteomics. 利用蛋白质组学技术对核桃子叶不定根发生期间表达的蛋白质进行了解析。
The optimized medium for rooting was 1/2 MS+ IAA 0.7mg/L+ AC 0.2mg/L+ ABT 0.2mg/L and the rhizogenesis rate reached to 100%. 最佳生根培养基为:1/2MS+IAA0.;7mg/L+AC(活性炭)0
During rhizogenesis（5d rooting culture）,33 proteins were up-regulated, 40 proteins down-regulated, and 108 protein maintained their level. 在不定根分化期(诱导5d)有33个蛋白质上调节表达，40个蛋白质下调节表达，108个蛋白质基本不变。
Therefore, it cries for technique innovation to solve this problem and for understanding the regulation mechanism underlying rhizogenesis. 因此，探索新方法、突破无性繁殖难关，深入研究不定根发生机制、根原基发生的调控规律显得尤为必要和迫切。
The propagation difficulty of walnut is presented as poor rhizogenesis, low and instable graft survival rate, low multiple efficiency and so on and so forth. 核桃无性繁殖难表现在：常规扦插很难生根、嫁接成活率不稳定、繁殖系数低等。
