In this paper, immobilized thermolysin was prepared by using chitosan as carrier,and glutaraldehyde as crosslinking agent. 本文报道了以甲壳胺为载体,采用戊二醛交联的方法将嗜热菌蛋白酶进行固定化。
In this study, zein, a very abundant and cheap crop in China was hydrolyzed by thermolysin, chymotrypsin, trypsin and alkaline protease for processing antihypertensive peptides. 本实验以我国来源丰富且价格低廉的玉米蛋白为原料,选用嗜热菌蛋白酶、胰凝乳蛋白酶、胰蛋白酶和碱性蛋白酶等4种酶来水解玉米蛋白制备降血压肽。
Isolation and culture of human intestinal epithelial cells using dissociating enzyme thermolysin 用嗜热菌蛋白酶进行人肠上皮细胞分离培养
Perreault N, Jean-Francois B.Use of the dissociating enzyme thermolysin to generate viable human normal intestinal epithelial cell cultures.Exp Cell Res 1996; 张道杰;蒋建新;陈永华;熊健琼;段朝霞;朱佩芳.;用嗜热菌蛋白酶进行人肠上皮细胞分离培养