AIM: To clone human IL-24 (hIL-24) gene and construct its eukaryotic expression vector, then transfect it into Ca Ski cells to express hIL-24 protein. 目的:克隆人IL-24基因并构建真核表达载体;转染Ca Ski细胞进行真核表达.
Methods:NPC cell line (CNE1) as target,Electroporation was used to transfect EBV-LMP gene and the vector into CNE1 cells. 方法：以人高分化鼻咽癌细胞株(CNE1)为对象，采用电穿孔基因转染技术，将重组EBV-LMP表达质粒转染CNE1细胞。
The chemotaxis and calcium mobil i zation assay were used for examining bioactivities of the cells stably transfect ed with CCR4. 通过基因转染技术获得稳定转染细胞株，利用趋化实验、钙流实验证实稳定转染细胞株能有效表达CCR4并具有生物功能。
The aim of gene therapy is to transfect DNA into target cells, in which ultilizing antisense oligonucleotides is a main method. 基因治疗是将DNA转染进入目的细胞,修复遗传错误或产生治疗因子。反义寡核苷酸的应用是基因治疗的主要手段之一。
Conclusion: jetPEI-RGD can efficiently transfect ASODN for inhibiting proliferation effect and inducing apoptosis on caco-2 cells in vitro. 结论：jetPEI-RGD介导的bcl-2反义核酸能抑制caco-2细胞增殖和诱导细胞凋亡。
Objective: The aim was to construct the recombinant of neuro related cell adhesion molecule(NrCAM) and immunoglobin Fc fragment and transfect it into insect cells. 目的 :构建神经原相关的细胞粘附分子 (NrCAM )和免疫球蛋白Fc片段融合基因的重组质粒并通过Baculovirus载体转染到昆虫细胞。
