In this study a Multiplex PCR method was developed for the rapid detection of genes encoding Shiga toxins 1 and 2 (stxl and stx2), intimin (eaeA) and H7 flagellin antigen (fliC). H7抗原由fliC基因编码，是EHEC 0157:H7的血清学标志之一。
This review focused on the research progress on bacterial flagella,including staining methods,the structure and function of flagella gene,as well as immunegenicity.of flagellin. 从细菌鞭毛的结构以及它在细菌致病性和免疫中的作用最新研究进展加以概述,以供细菌研究者参考。
Bacterial flagella are much simpler than undulipodia, being hollow cylinders about 15 nm in diameter, consisting of subunits of a protein (flagellin) arranged in helical spirals. 细菌鞭毛比UNDULIPODIA简单很多，它是由含有螺旋排列的蛋白质亚单位（鞭毛蛋白）组成的，直径约15nm的中空圆柱体。
Western blotting analysis showed the purified flagellin strip was located at 52kD location,the protein that negatively stained with phosliolungstic appeared filaceous in scanning electron microscope(SEM). 纯化的鞭毛蛋白经Western Blot 检测,在52kD 处见到清楚的蛋白条带,磷钨酸负染扫描电镜观察到蛋白呈细丝状。
This study was therefore undertaken to determine flagellin in serum, BALF and lung homogenate of rat after cecal ligation and puncture (CLP), and evaluate the relationship between flagellin concentrations and ALI induced by sepsis. 目的 本实验旨在采用盲肠结扎穿孔(cecal ligation and puncture,CLP)所致脓毒症诱发的大鼠ALI模型,观测脓毒症肺损伤大鼠鞭毛蛋白含量的变化,来探讨脓毒症肺损伤时鞭毛蛋白含量变化与肺损伤发生及损伤程度的关系,为阐明ALI发病机理及其防治提供理论及实验依据。
To detect the variability and its pathogenecity of Helicobacter pylori(H.pylori),fifty- two biopsy specimens and 18 strains of H.pylori were performed PCR for 16S rRNA gene and flagellin A gene(flaA). PCR-RFLP and PCR-RFLP-SSCP were also performed. 我们对52例胃粘膜活检标本和18株 Hp 临床分离株进行 Hp 特异的16S rRNA 基因和鞭毛素 A 基因 PCR 扩增和 PCR-RFLP 及 PCR-RFLP-SSCP 分析,以检测 Hp 鞭毛素 A 基因的变异性,进而研究其与 Hp 致病性的关系。
